Direct Acid Methylation for Extraction of Fatty Acid Content from Microalgae Cells
By: Benjamin D. Frigo-Vaz, Ping Wang
Abstract:
Direct acid methylation was examined as a means for both analysis of fatty acid content in microalgal cells and biodiesel production without pretreatment. Microalgal cells of Chlamydomonas reinhardtii and Dunaliella tertiolecta were prepared and examined. It appeared that direct acid methylation extracted higher fatty acid content than the solvent-based Soxhlet extraction process. It also revealed that the latter was prone to extract a significant amount of nonlipid hydrophobic impurities, including hydrophobic proteins and phytol-type compounds, while direct methylation produces essentially pure ester product. This work demonstrates that direct acid methylation provides superior fatty acid extraction, promising an efficient process for either quantification of lipid content or production of biodiesel.
Link to Applied Biochemistry and Biotechnology Journal for book.
Flora ecology maintenance, watershed restoration, public health restoration, bring them both together, Naturally healthy city planning for a healthy city, organic food given to the poor in farmers market, feeding the poor with healthy food, vegetable gardens, planting, green living in the home, redwood forest, amazon basin maintenance, oxygen from trees, oxygen most important substance for life and the brain, sustainable ecology and sustainable medicine for a healthy planet and its habitats, exercise, soccer, games, yoga, meditation, walks in nature, the beach, surfing, waterfall, water holes, biking, rollerblading, creek restoration, ecology, hydrology, watersheds, health information technology for the healthy ones to staying healthy. Peaceful warrior, bringing harmony where chaos reigns, health where sickness rules.
Ben BioChem 
Leave a Reply